Copenhagen BioProcessing Technology Seminar - 9 October 2013

Please join us for this FREE event to learn more about the latest advancements in bioproduction technologies.

Subject to Change

Time		Session
8:30 a.m. - 10:00 a.m.		Registration & Continental Breakfast
10:00 a.m. - 10:45 a.m.		Keynote Speaker
10:45 a.m. - 11:30 a.m.		TBD
11:30 a.m. - 12:00 p.m.		Metabolic Pathway Design™ Process: Cell Culture Media and Process Optimization
12:00 p.m. - 12:30 p.m.		HyCell™ CHO Medium: Targeted for High Cell Density and Productivity
12:30 p.m. - 1:30 p.m.		Lunch
1:30 p.m. - 2:00 p.m.		TBD
2:00 p.m. - 2:30 p.m.		Single-Use Bioreactor: Turnkey and Open-Architecture
2:30 p.m. - 3:00 p.m.		Break
3:00 p.m. - 3:30 p.m.		HyPerforma™ Single-Use Mixer DS 300
3:30 p.m. - 4:00 p.m.		Hands on session: S.U.M. DS 300
4:00 p.m. - 4:30 p.m.		Prepare for CMC tour
4:30 p.m. - 5:00 p.m.		Travel to CMC
5:00 p.m. - 7:00 p.m.		Reception and facility tour by CMC
7:00 p.m.		Return to hotel

Copenhagen BioProcessing Technology Seminar – 9 October 2013

Radisson Blu Scandinavia Hotel - Copenhagen

A sleek, modern retreat within easy walking and cycling distance of all the city has to offer, the Radisson Blu sits south across the Stadsgraven from trendy Christianshavn, bursting with cafés, canals and dramatic architecture. This city hotel gives Copenhagen guests easy access to Strøget, Tivoli Gardens and Town Hall Square, each just two kilometres away. The hotel lies near Copenhagen International Airport, and the subway station is within walking distance.

An ideal option for business travelers, this hotel near Copenhagen Kastrup Airport provides a stress-free, 10-kilometre commute. With on-site meeting facilities and sophisticated dining options.

To receive a special seminar rate for your accommodations, please click here, or mention “Thermo Fisher Tradeshow” when booking.

Radisson Blu Scandinavia Hotel, Copenhagen – Amager Boulevard 70 – DK-2300 – Copenhagen – Denmark
Phone: +45 3396 5000

CMC Biologics – Special Tour

CMC Biologics is leading the industry among CMO's in reliability, technical excellence, and quality – Right and On Time. With facilities in the USA and Europe, the Company is a global CMO that provides fully integrated biopharmaceutical development and manufacturing solutions to clients around the world. The Company has proven expertise in delivering custom solutions for the scale-up and cGMP manufacture of protein-based therapeutics for pre-clinical, clinical trials and commercial production. The Company's wide range of integrated services includes cell line development, bioprocess development, formulation and comprehensive analytical testing. Clients can also benefit from CMC Biologics' proprietary CHEF1® expression system for mammalian production. CMC Biologics has fully segregated microbial fermentation and mammalian cell culture suites and offers both fed-batch and perfusion production processes. CMC Biologics is located in Copenhagen, Denmark; Seattle, Washington; and Berkeley, California. More detailed information can be found at www.cmcbiologics.com.

Agenda Abstracts

Metabolic Pathway Design Process: Cell culture media and process optimization approaches for optimal biotherapeutic production

Clonal variation in production clones drives unique nutritional demands in order to achieve optimal bioproduction. A media optimization approach that has the ability to thoroughly evaluate the nutrient demands specific to that clone for improved productivity is critical. Design of experiment factorial strategy along with our Thermo Scientific™ Metabolic Pathway Design™ process has been successfully used to alter formulations in order to reduce the effect of limiting factors and drive key metabolic pathways in order to minimize waste and maximize productivity. High-throughput screening (HTS) equipment facilitated the development and testing of the media formulations. Development and comparative analysis were performed in 96-deepwell plates with subsequent scale-up and comparison of promising formulations in shake flasks and bioreactor studies. Results revealed exceptionally similar trends in both cell density and product quantity, validating the HTS plate design and Metabolic Pathway Design process. This strategy has been successfully utilized to develop clone specific media formulations and supplement feeds. Case studies will discuss specific optimization work.

 

HyCell CHO Medium: Targeted for high cell density and productivity across a broad variety of CHO clones

The clonal variation observed in various bioproduction CHO clones has increased demands for a versatile high performing cell culture medium. The Thermo Scientific™ Metabolic Pathway Design™ process and design of experiments approach to mixture design were utilized to develop Thermo Scientific™ HyCell™ CHO medium that will work well for the majority of the CHO clones being used in bioprocess production. High-throughput screening allowed for inclusion of more individual cell lines, increased component factor-levels and mixture design treatments, and higher number of replicates. Developmental work was performed at the 96-deepwell scale, with subsequent scale-up and comparison of promising treatments at shake flask and bioreactor levels. The HyCell CHO medium development approach and comparative performance will be presented.

 

Turnkey open architecture

The Thermo Scientific™ HyPerforma™ Single-Use Bioreactor is the market leading bioreactor in stirred tank design. We have hundreds of installed systems worldwide used to grow numerous cell lines in a variety of processes. The bioreactor consists of a stainless steel outer container and a single-use bag assembly. The system can be ordered either in an open architecture format or as a turnkey system. The open architecture design allows customers to: integrate with any control platform on the market; plug-and-play through standard disposable connectors; avoid the costs of investing in an additional control system; and have the ultimate flexibility to choose the control platform they want. Get your turnkey solution using the control platform of your choice for either cGMP or non-cGMP applications.

 

HyPerforma S.U.M. DS 300

Single-use mixers offer significant advantages by eliminating the need for cleaning and sterilizing, reducing the risk of cross-contamination, and reducing cycle times. The Thermo Scientific™ HyPerforma™ Single-Use Mixer DS 300 (S.U.M. DS 300) provides enhanced mobility, interoperability, and modularity previously unavailable from mixing solutions. It can dock into multiple mixing barrels in 50, 100, 200 and 300L sizes. Single-use bioprocessing container bags were designed for each drum size. It is designed to efficiently mix powder-to-liquid or liquid-to-liquid solutions at different volumes for a wide variety of bioprocess applications: hydration of powdered media and buffers; sterile mixing of buffer solutions; cell culture media with sera and reagents or other process fluids; and pooling sterile liquid fractions. The HyPerforma S.U.M. DS 300 system adds value to your process by mixing and storing in multiple drums economically while maintaining a smaller footprint. You can now achieve greater process flexibility by doing more with less.

 

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